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. 2014 Nov 25;9(11):e110454. doi: 10.1371/journal.pone.0110454

Table 1. Primer sets for qRT-PCR analysis.

Gene Accession No. Primer sequences (5′-3′) Length (bp) E (%)* R2,**
EF1A AY219737 F: AGAATGGACAAACCCGTGAA 104 104.8 0.9989
R: GCTGTATGGTGGTTCAGTAGAG
Tublin NM_001190398 F: TTGGTACACTGGTGAAGGTATG 103 103.5 0.9980
R: AGCGGTAGCTTCTTGGTATTG
NADH NM_001162323 F: CGAGGAGAACATGCTCTTAGAC 93 104.6 0.9989
R:GATAGCTTGGGCTGGACATATAG
RPL12 NM_001126171 F: AAGGCTACATCTGACTGGAAAG 101 108.0 0.9992
R: ACCAATGATGATGCAGAAGGA
SDHB NM_001162436 F: CTGAATTCCTGTGGACCTATGG 90 102.9 0.9963
R: ACGGCAAGAACGCCTAAA
18S X62623 F: CCGCGAAACCGTCATTAAATC 101 103.9 0.9921
R: GGAACTCTGTCGGCATGTATTA
28S S50426 F: CGGGTGGTAAACTCCATCTAAC 118 97.2 0.9943
R:CGAGCGGTTTCACGTTCTTA
16S FJ411411 F:AGAAACCAACCTGGCTTACAC 121 109.9 0.9989
R:TTGCGACCTCGATGTTGAATTA
v-ATPAase NM_001126156 F: CTTCTCTGCTGAGTGCTGTT 90 99.4 0.9931
R: GCCATCACGACGACTGATTA
Actin NM_001126200 F: CGTTACCAACTGGGACGATATG 111 105.8 0.9985
R: GGGTTCAATGGAGCTTCTGTTA
TATA NM_001162717 F: CACCTAATGTCACCAGCCTATT 123 106.9 0.9992
R: TGTGTCCAAGGCGTTCTAAG

“*”: PCR efficiency (calculated from the standard curve).

“**”: Regression coefficient.