Table 1. Primer sets for qRT-PCR analysis.
Gene | Accession No. | Primer sequences (5′-3′) | Length (bp) | E (%)* | R2,** |
EF1A | AY219737 | F: AGAATGGACAAACCCGTGAA | 104 | 104.8 | 0.9989 |
R: GCTGTATGGTGGTTCAGTAGAG | |||||
Tublin | NM_001190398 | F: TTGGTACACTGGTGAAGGTATG | 103 | 103.5 | 0.9980 |
R: AGCGGTAGCTTCTTGGTATTG | |||||
NADH | NM_001162323 | F: CGAGGAGAACATGCTCTTAGAC | 93 | 104.6 | 0.9989 |
R:GATAGCTTGGGCTGGACATATAG | |||||
RPL12 | NM_001126171 | F: AAGGCTACATCTGACTGGAAAG | 101 | 108.0 | 0.9992 |
R: ACCAATGATGATGCAGAAGGA | |||||
SDHB | NM_001162436 | F: CTGAATTCCTGTGGACCTATGG | 90 | 102.9 | 0.9963 |
R: ACGGCAAGAACGCCTAAA | |||||
18S | X62623 | F: CCGCGAAACCGTCATTAAATC | 101 | 103.9 | 0.9921 |
R: GGAACTCTGTCGGCATGTATTA | |||||
28S | S50426 | F: CGGGTGGTAAACTCCATCTAAC | 118 | 97.2 | 0.9943 |
R:CGAGCGGTTTCACGTTCTTA | |||||
16S | FJ411411 | F:AGAAACCAACCTGGCTTACAC | 121 | 109.9 | 0.9989 |
R:TTGCGACCTCGATGTTGAATTA | |||||
v-ATPAase | NM_001126156 | F: CTTCTCTGCTGAGTGCTGTT | 90 | 99.4 | 0.9931 |
R: GCCATCACGACGACTGATTA | |||||
Actin | NM_001126200 | F: CGTTACCAACTGGGACGATATG | 111 | 105.8 | 0.9985 |
R: GGGTTCAATGGAGCTTCTGTTA | |||||
TATA | NM_001162717 | F: CACCTAATGTCACCAGCCTATT | 123 | 106.9 | 0.9992 |
R: TGTGTCCAAGGCGTTCTAAG |
“*”: PCR efficiency (calculated from the standard curve).
“**”: Regression coefficient.