Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Aug 23;11:148. doi: 10.1186/s12974-014-0148-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Yang et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

CNPase expression was increased in primary cultured microglia following lipopolysaccharide treatment. (A) Note the change in external morphology of primary microglial cells bearing long extending and stout processes after lipopolysaccharide (LPS) treatment (Ab) in comparison with the control (Aa) under the phase contrast microscope. (B) Confocal images showing CNPase expression (Bb, Be; red) in primary microglia labeled with lectin (Ba, Bd; green) and DAPI (blue) in both control and LPS treatment for 24 hours. CNPase immunofluorescence intensity is enhanced after LPS treatment (Bf) in comparison with the control (Bc). (C) CNPase mRNA expression in control and LPS activated primary microglia. LPS stimulated primary microglial cells show a significant upregulation of CNPase mRNA in comparison with the control cells. ***P < 0.001. The values represent the mean ± SD in triplicate. Scale bars = 100 μm (A) and 20 μm (B). CNPase, 2′,3′-cyclic nucleotide 3′-phosphodiesterase; DAPI, 4′,6- diamidino-2-phenylindole.