Figure 1. Binding affinities and in vivo activities of human insulin and [GlnB22]-insulin.

(A) Inhibition of the binding of human [¹²⁵I]-insulin to the plasma membrane of IM-9 cells by human insulin (blue) and [GlnB22]-insulin (red). (B) Insulin tolerance test of human insulin (blue) and [GlnB22]-insulin (red). Values are mean ±S.E., n = 10/group. ***p<0.01 compared to human insulin-treated group. Δ c_M is the decrease in molar concentration of blood glucose in mmol/l adjusted for the impact of conditions during testing (represented by the application of saline).