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. 2014 Nov 25;9(11):e113129. doi: 10.1371/journal.pone.0113129

Figure 2. MMP-11 expression in oral cancer cell lines and the MMP-11 knockdown in SCC9 cells reduce cell migration.

Figure 2

(A) Western blot analysis showing the expression of MMP-11 protein in five oral cancer cell lines. (B) MMP-11 mRNA levels in five oral cancer cell lines were determined by quantitative real-time PCR using β-actin as an endogenous control. Results are shown as mean ± SD of three independent experiments. (C) Endogenous MMP-11 expression is silenced by infection with 4 different shRNA constructs (#1 and #2, #3 and #4) for MMP-11 in SCC9 oral cancer cells. Knockdown efficiency was analyzed by Western blotting. Results showed an approximately 80% reduction of #1 shRNA of MMP-11. *p<0.05. (D) MMP-11 Knockdown efficiency in SCC9 cell was confirmed by quantitative real-time PCR. (E) Detection of cell migration ability by infection with shLuc or shRNA of MMP-11 in SCC9 cell. Results shown that MMP-11 shRNA significantly reduced the migration of SCC9 cells. *p<0.05.