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After HT-1080 cells undergo serum deprivation for 48 hr, the cells revive by replenishing serum for 22 hr. A. Regular growing cells, B. cell cycle arrested cells and C. cell survival cells were harvested, fixed and treated with propidium iodide for DNA staining, and then analyzed on flow cytometry. Three arbitrary groups of cells on the map were labeled as i, ii and iii.
