Table 1. Mutants identified through Constrictor modifications in the ethylene core network (f_minor = 0.8, f_major = 0.2).

Strain	Reactions Modified (Individually)	Expression State	Ethylene Yield	% ΔYield (with respect to baseline)	Growth Rate (hr-1)
Baseline	-	-	0.35	-	0.3
Upper limit on glucose uptake
Mutant A	GLCpts	Minor (80% of baseline)	0.38	+9%	0.3
Mutant B	GAPD, PGK	Minor (80% of baseline)	0.37	+6%	0.3
Not viable	Ex_nh4(e)†, NH4t†, GLCpts†, RPI*, Ex_pi(e)*, PIt2r*	†Major (20% of baseline) *Either Major or Minor	0	−100%	0
Fixed glucose uptake
Mutant C	GAPD, PGK	Minor	0.18	−48%	0.45
Mutant D	ENO, PGM	Minor	0.20	−43%	0.43
Not viable	ENO†, GAPD†, PFK†, PGK†, FBA†, PGM†, TPI†, Ex_nh4(e) †, NH4t†, RPI*, Ex_pi(e)*, PIt2r*, GLCpts*	†Major *Either Major or Minor	0	−100%	0