Table 2. Highest ethylene yielding strains obtained via Constrictor modification of individual reactions in three E. coli network models.
Strain | Reactions Modified (Individually) | Expression State | Ethylene Yield | % Δ Yield (with respect to baseline) | Growth Rate (hr−1) |
In core model: | |||||
Baseline | - | - | 0.35 | - | 0.30 |
Mutant E | ENO, PGM | 50% of baseline | 0.44 | +25% | 0.30 |
Mutant F | FBA, PFK, TPI | 50% of baseline | 0.43 | +23% | 0.30 |
In iAF1260: | |||||
Baseline | - | - | 0.35 | - | 0.30 |
Mutant G | GLCtex | 70% of baseline | 0.41 | +18% | 0.30 |
Mutant H | GAPD, PGK | 40% of baseline | 0.39 | +12% | 0.30 |
In iJO1366: | |||||
Baseline | - | - | 0.43 | - | 0.30 |
Mutant I | GAPD, PGK | 30% of baseline | 0.51 | +18% | 0.30 |
Mutant J | GAPD, PGK | 20% of baseline | 0.46 | +5% | 0.30 |