Table 3. Maximum and minimum ethylene yield changes resulting from Constrictor modification of functional classes (f_minor = 0.8, f_major = 0.2).

		Core Model		iAF1260		iJO1366
Functional Class	Reactions Modified (Simultaneously)	Max % ΔYield	Min % ΔYield	Max % ΔYield	Min % ΔYield	Max % ΔYield	Min % ΔYield
Amino acid + Glutamate Metabolism + Anaplerotic reactions	ASPTA, P5CD, GLNS, GLUDy, PPC	−26%	−83%	Not viable	Not viable	Not viable	Not viable
L-arginine biosynthesis	ACGK, ACGS, ACODA, ACOTA, AGPR, ARGSL, ARGSS, CBPS, OCBT	−20%	−80%	−21%	−84%	−21%	−83%
Exchange	EX_ac(e), EX_co2(e), EX_for(e), EX_etoh(e), EX_h2o(e)	−25%	−99%	−25%	−62%	−27%	−95%
Glycolysis	ENO, FBA, GAPD, PFK, PGI, PGK, PGM, PYK, TPI	+6%	−92%	−3%	−45%	+5%	−26%
Oxidative Phosphorylation	ADK1, ATPS4r, CYBD, PPKr, SUCDi, THD2, FRD7, NADH16	−21%	−86%	−0%	−2%	−1%	−6%
Pentose Phosphate Pathway	RPE, TALA, TKT1, TKT2	0%	−0.1%	−0.1%	−1%	0%	−0.2%
Pyruvate Metabolism	ACALD, ACKr, ALCD2x, PFL, PTAr	−2%	−8%	−1%	−4%	−2%	−8%
TCA Cycle	ACONTa, ACONTb, CS, FUM, ICDHyr, MDH	−21%	−85%	−21%	−83%	−21%	−84%