Table 1. Some parameters of miRNAs and the partition of monomer and dimer formation in concentration dependence.
miR-21 | miR-93 | miR-296 | |
Free energy of dimer [kcal/mol] | −13.458649 | −13.541779 | −15.170716 |
Free energy of monomer [kcal/mol] | −1.980884 | −3.900905 | −4.140155 |
Concentration in which miRNA dimer and monomer are in equilibrium [M] | 163 nM | 51 µM | 8.1 µM |
Concentration in which 50% of molecules form dimer [M] | 135 nM | 60.7 µM | 9.27 µM |
The partition of monomer fraction in 10 nM miRNA | 88.5% | ∼100% | ∼100% |
The partition of monomer fraction in 0.7 mM miRNA | 1.9% | 31.5% | 14.5% |
The partition of monomer fraction in cellular miRNA concentration (166 pM) | ∼100% | ∼100% | ∼100% |