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. 2014 Dec 1;25(24):3861–3869. doi: 10.1091/mbc.E14-04-0863

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© 2014 Ribeiro, D'Ambrosio, et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 4: — Cells expressing integrin have different motility behaviors. Focal adhesion dynamic behavior requires FA maturation. (A) Example of a focal adhesion (labeled with p130Cas-GFP) turnover in α-PS⁺ cell plated on vitronectin: assembly (increasing intensity, blue arrowhead), maturation (constant intensity, red arrowhead), and disassembly (decreasing intensity, green arrowhead). Time: min; scale bar: 1 μm. (B and C) Still images from a time-lapse sequence of an α-PS⁺ cell showing motility (B) and an α-PS⁺ cell that is stationary (C) show clusters of FA appearing and disappearing in a synchronous manner at the perimeter (Supplemental Movie 1 and 2 correspond to the stills shown). Time: min; scale bar: 5 μm. (D) Kymograph of a cell slice (red dashed rectangle in C) shows the appearance and disappearance of FA at the bottom and top of the cell in an out-of-phase manner. (E–G) Kymograph of representative cell slices after FAK (E), Pak3 (F), and myosin II heavy-chain (G) depletion showing focal adhesion dynamics at opposite sides of a cell (see Figure 4D for more details). Scale bar: 5 μm.