Table 3. Deamidated route: estimates of NMNAT and NADS physiological activities.

	NMNAT total ratea	NADS rateb	Combined values
	nmol/min/g	nmol/min/g	nmol/min/g
skeletal muscle	∼0.05	≤0.001	≤0.001
lung	∼0.02	≤0.001	≤0.001
kidney	∼0.007	∼0.007	∼0.007
brain	∼0.001	∼0.0006	∼0.0008
spleen	∼0.02	∼0.01	∼0.02
small intestine	∼0.03	∼0.07	∼0.05
heart	∼0.05	∼0.02	∼0.04
liver	∼0.17	∼0.23	∼0.20
blood	∼0.0005	∼0.001	∼0.001

^aSum of the three NMNAT isozyme activities individually calculated as in Table 2 but versus the NaMN substrate (K _m values used are reported in ref [19]).

^bValues are calculated from the steady-state kinetic equation (3) shown in Methods, using the NADS activities measured at saturating substrates (Figure 3A) as V _max values, the NaAD, ATP, and glutamine levels (nmol/g, Figure 4) as micromolar concentrations, and the K _m values from [43].