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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: J Cell Physiol. 2015 Mar;230(3):595–602. doi: 10.1002/jcp.24777

Figure 2. LTD4 and SCF synergistically phosphorylate c-Kit.

Figure 2

c-Kit phosphorylation was analyzed by western blotting in LAD2 cell lysates (A) stimulated with 500 nM of LTC4, LTD4 and LTE4 in presence or absence of 100ng/ml SCF for 15 minutes. (B) Phospho-c-Kit levels upon stimulation with 500 nM LTD4 and/or of 100 ng/ml SCF for 15 minutes with/without MK571 (1µM) pre-treatment (30 min). (C) Densitometric analysis of phospho-c-Kit levels upon SCF and /or LTD4 stimulation in the presence or absence of MK571 (1µM). The data represents mean ± SEM of three separate experiments. The significance was tested using one way ANOVA and post-hoc analysis *P<0.05.