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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: Nat Genet. 2014 Nov 2;46(12):1363–1371. doi: 10.1038/ng.3138

Figure 3.

Figure 3

Experimental Validation of Wild-type and Mutated Protein Level Interactions. (a) Quantitative co-immunoprecipitation signals of GCSAM to partner proteins show excellent experimental reproducibility (ρ = 0.99) and a high correlation with MSM/P predictions (ρ = 0.80). (b) A1346V mutated IGF1R exhibits higher affinity to the PIK3R1-N, PIK3R1-C, and PIK3R1-NC SH2 domains (p = 6.2 × 10−5, p = 9.0 × 10−3, and p = 5.9 × 10−5, respectively, using one-sided T-test) as predicted by MSM/P. Error bars represent the standard error of five biological replicates.