Figure 2.

Age-dependent increases in astrocytic aerobic metabolism. (A) Representative figure of changes in astrocytic aerobic metabolism with age. Primary astrocytes were cultured on Seahorse XF-24 plates, and oxygen consumption rate (OCR) was measured in the presence of pyruvate as the solely important substrate. A more comprehensive and quantitative comparison of astrocytic aerobic metabolism in the presence of different substrates was shown in Table1. (B) Age-dependent increase in mitochondrial biogenesis was determined by the ratio of COX3/18SrDNA, assayed by real-time PCR. (C) Age-dependent increase in mitochondrial membrane potential was determined by TMRM staining using FACS. (D) Quantification of the increase in mitochondrial membrane potential with age. *P < 0.05.