Figure 3.

Age-dependent increases in Astrocytic H₂O₂ generation and NOX expression. (A) H₂O₂ release rate in the astrocytic medium was determined by Amplex Red assay. Primary astrocytes were treated with antimycin A, deprenyl, or DPI at 50 μm for 1 h to determine the contribution to H₂O₂ generation from different subcellular sources. (B) Increased DPI-sensitive H₂O₂ release rate with age. DPI-sensitive H₂O₂ release rate was defined by subtraction of H₂O₂ release rate with DPI treatment from the basal release rate. Expressions of (C) NOX2 and (D) NOX4 were determined by Western blot. *P < 0.05.