Table 1.
Primer Sequences Used for Real Time Quantitative PCR.
| Gene | Accession No. | Sequence (5’ to 3’) | Amplicon size, bp |
|---|---|---|---|
| GAPDH | NM_008084.3 | 5’-CCTGGAGAAACCTGCCAAGTAT | 133 |
| 5’-AGAGTGGGAGTTGCTGTTGAAG | |||
| TRAP | NM_001111036.1 | 5’-TGACAAGAGGTTCCAGGA | 318 |
| 5’-AGCCAGGACAGCTGAGTG | |||
| NFATc1 | NM_001164111.1 | 5’-GCATCACAGGGAAGACCGTGTC | 153 |
| 5’-GAAGTTCAATGTCGGAGTTTCTGAG | |||
| CatK | NM_007802.4 | 5’-ACCGGGGTATTGACTCTGAA | 190 |
| 5’-GAGGTCAGGCTTGCATCAAT | |||
| Fra-2 | NM_008037.4 | 5’-GATCAAGACCATTGGCACCAC | 79 |
| 5’-GCGACGCTTCTCCTCCTCT | |||
| Beclin1 | NM_019584.3 | 5’-AGCTCAGTACCAGCGGGAGT | 147 |
| 5’-TGGAAGGTGGCATTGAAGAC | |||
| Atg5 | NM_053069.5 | 5’-GCCTATATGTACTGCTTCATCCA | 267 |
| 5’-CATTTCAGGGGTGTGCCTTCA | |||
| Atg7 | NM_028835.4 | 5’-GCTGTGGAGCTGATGGTCTC | 166 |
| 5’-CCAGGCTGACAGGAAGAACA | |||
| Atg12 | NM_026217.3 | 5’-AACAAAGAAATGGGCTGTGG | 198 |
| 5’-TTGCAGTAATGCAGGACCAG | |||
| LC3 | NM_026160.4 | 5’-CGGAGCTTTGAACAAAGAGTG | 279 |
| 5’-TCTCTCACTCTCGTACACTTC |
Atg, autophagy-related genes; CatK, Cathepsin K; LC3, microtubule-associated protein 1 light chain 3; NFATc1, nuclear factor of activated T-cells, cytoplasmic 1 (NFATc1); TRAP, tartrate-resistant acid phosphatase.