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. 1987 May;79(5):1538–1541. doi: 10.1172/JCI112986

Regulation of inositol 1,4,5-trisphosphate kinase activity after stimulation of human T cell antigen receptor.

J B Imboden, G Pattison
PMCID: PMC424432  PMID: 3494750

Abstract

Inositol 1,4,5-trisphosphate (Ins-1,4,5-P3), a Ca2+-mobilizing messenger, can be phosphorylated by a cytoplasmic kinase, yielding inositol 1,3,4,5-tetrakisphosphate (Ins-1,3,4,5-P3). We observed that stimulation of the antigen receptor on a malignant human T cell line, Jurkat, led to substantial, sustained increases in Ins-1,4,5-P3 and InsP4. The Ins-1,4,5-P3 kinase partially purified from resting Jurkat cells had a maximum velocity (Vmax) of 0.09 nmol/min/mg protein and an apparent Michaelis constant (Km) of 0.2 microM. When the kinase was partially purified 10 min after stimulation of the antigen receptor or after the addition of phorbol myristate acetate, the Vmax was increased twofold. The activity of the Ins-1,4,5-P3 kinase obtained from either resting or stimulated Jurkat cells was enhanced in vitro by increasing the concentration of free Ca2+ from 0.1 to 0.5 microM. These results indicate that the activity of the Ins-1,4,5-P3 kinase is regulated as a consequence of stimulating the T cell antigen receptor.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Berridge M. J., Irvine R. F. Inositol trisphosphate, a novel second messenger in cellular signal transduction. Nature. 1984 Nov 22;312(5992):315–321. doi: 10.1038/312315a0. [DOI] [PubMed] [Google Scholar]
  2. Castagna M., Takai Y., Kaibuchi K., Sano K., Kikkawa U., Nishizuka Y. Direct activation of calcium-activated, phospholipid-dependent protein kinase by tumor-promoting phorbol esters. J Biol Chem. 1982 Jul 10;257(13):7847–7851. [PubMed] [Google Scholar]
  3. Connolly T. M., Lawing W. J., Jr, Majerus P. W. Protein kinase C phosphorylates human platelet inositol trisphosphate 5'-phosphomonoesterase, increasing the phosphatase activity. Cell. 1986 Sep 12;46(6):951–958. doi: 10.1016/0092-8674(86)90077-2. [DOI] [PubMed] [Google Scholar]
  4. Downes C. P., Mussat M. C., Michell R. H. The inositol trisphosphate phosphomonoesterase of the human erythrocyte membrane. Biochem J. 1982 Apr 1;203(1):169–177. doi: 10.1042/bj2030169. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hansen C. A., Mah S., Williamson J. R. Formation and metabolism of inositol 1,3,4,5-tetrakisphosphate in liver. J Biol Chem. 1986 Jun 25;261(18):8100–8103. [PubMed] [Google Scholar]
  6. Imboden J. B., Shoback D. M., Pattison G., Stobo J. D. Cholera toxin inhibits the T-cell antigen receptor-mediated increases in inositol trisphosphate and cytoplasmic free calcium. Proc Natl Acad Sci U S A. 1986 Aug;83(15):5673–5677. doi: 10.1073/pnas.83.15.5673. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Imboden J. B., Stobo J. D. Transmembrane signalling by the T cell antigen receptor. Perturbation of the T3-antigen receptor complex generates inositol phosphates and releases calcium ions from intracellular stores. J Exp Med. 1985 Mar 1;161(3):446–456. doi: 10.1084/jem.161.3.446. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Imboden J. B., Weiss A., Stobo J. D. The antigen receptor on a human T cell line initiates activation by increasing cytoplasmic free calcium. J Immunol. 1985 Feb;134(2):663–665. [PubMed] [Google Scholar]
  9. Irvine R. F., Anggård E. E., Letcher A. J., Downes C. P. Metabolism of inositol 1,4,5-trisphosphate and inositol 1,3,4-trisphosphate in rat parotid glands. Biochem J. 1985 Jul 15;229(2):505–511. doi: 10.1042/bj2290505. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Irvine R. F., Letcher A. J., Heslop J. P., Berridge M. J. The inositol tris/tetrakisphosphate pathway--demonstration of Ins(1,4,5)P3 3-kinase activity in animal tissues. Nature. 1986 Apr 17;320(6063):631–634. doi: 10.1038/320631a0. [DOI] [PubMed] [Google Scholar]
  11. Kuno M., Goronzy J., Weyand C. M., Gardner P. Single-channel and whole-cell recordings of mitogen-regulated inward currents in human cloned helper T lymphocytes. Nature. 1986 Sep 18;323(6085):269–273. doi: 10.1038/323269a0. [DOI] [PubMed] [Google Scholar]
  12. Ledbetter J. A., June C. H., Martin P. J., Spooner C. E., Hansen J. A., Meier K. E. Valency of CD3 binding and internalization of the CD3 cell-surface complex control T cell responses to second signals: distinction between effects on protein kinase C, cytoplasmic free calcium, and proliferation. J Immunol. 1986 Jun 1;136(11):3945–3952. [PubMed] [Google Scholar]
  13. Meuer S. C., Acuto O., Hercend T., Schlossman S. F., Reinherz E. L. The human T-cell receptor. Annu Rev Immunol. 1984;2:23–50. doi: 10.1146/annurev.iy.02.040184.000323. [DOI] [PubMed] [Google Scholar]
  14. Meuer S. C., Hodgdon J. C., Hussey R. E., Protentis J. P., Schlossman S. F., Reinherz E. L. Antigen-like effects of monoclonal antibodies directed at receptors on human T cell clones. J Exp Med. 1983 Sep 1;158(3):988–993. doi: 10.1084/jem.158.3.988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Mills G. B., Cheung R. K., Grinstein S., Gelfand E. W. Interleukin 2-induced lymphocyte proliferation is independent of increases in cytosolic-free calcium concentrations. J Immunol. 1985 Apr;134(4):2431–2435. [PubMed] [Google Scholar]
  16. Molina y Vedia L. M., Lapetina E. G. Phorbol 12,13-dibutyrate and 1-oleyl-2-acetyldiacylglycerol stimulate inositol trisphosphate dephosphorylation in human platelets. J Biol Chem. 1986 Aug 15;261(23):10493–10495. [PubMed] [Google Scholar]
  17. Nisbet-Brown E., Cheung R. K., Lee J. W., Gelfand E. W. Antigen-dependent increase in cytosolic free calcium in specific human T-lymphocyte clones. Nature. 1985 Aug 8;316(6028):545–547. doi: 10.1038/316545a0. [DOI] [PubMed] [Google Scholar]
  18. Nishizuka Y. Studies and perspectives of protein kinase C. Science. 1986 Jul 18;233(4761):305–312. doi: 10.1126/science.3014651. [DOI] [PubMed] [Google Scholar]
  19. Oettgen H. C., Terhorst C., Cantley L. C., Rosoff P. M. Stimulation of the T3-T cell receptor complex induces a membrane-potential-sensitive calcium influx. Cell. 1985 Mar;40(3):583–590. doi: 10.1016/0092-8674(85)90206-5. [DOI] [PubMed] [Google Scholar]
  20. Shapiro D. N., Adams B. S., Niederhuber J. E. Antigen-specific T cell activation results in an increase in cytoplasmic free calcium. J Immunol. 1985 Oct;135(4):2256–2261. [PubMed] [Google Scholar]
  21. Stewart S. J., Prpic V., Powers F. S., Bocckino S. B., Isaacks R. E., Exton J. H. Perturbation of the human T-cell antigen receptor-T3 complex leads to the production of inositol tetrakisphosphate: evidence for conversion from inositol trisphosphate. Proc Natl Acad Sci U S A. 1986 Aug;83(16):6098–6102. doi: 10.1073/pnas.83.16.6098. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Weiss A., Imboden J., Shoback D., Stobo J. Role of T3 surface molecules in human T-cell activation: T3-dependent activation results in an increase in cytoplasmic free calcium. Proc Natl Acad Sci U S A. 1984 Jul;81(13):4169–4173. doi: 10.1073/pnas.81.13.4169. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Weiss A., Stobo J. D. Requirement for the coexpression of T3 and the T cell antigen receptor on a malignant human T cell line. J Exp Med. 1984 Nov 1;160(5):1284–1299. doi: 10.1084/jem.160.5.1284. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Wollheim C. B., Biden T. J. Second messenger function of inositol 1,4,5-trisphosphate. Early changes in inositol phosphates, cytosolic Ca2+, and insulin release in carbamylcholine-stimulated RINm5F cells. J Biol Chem. 1986 Jun 25;261(18):8314–8319. [PubMed] [Google Scholar]

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