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WT cells expressing both Jen1-GFP and Sec7-mCh, and vps52Δ cells expressing only Jen1-GFP were grown 4 hr in lactate medium and observed simultaneously for 10 min of glucose addition and 20 min after glucose removal. Co-localization between Jen1-GFP and Sec7-mCh in WT cells is indicated by white arrows in the merge panel (second on the right). See also Figure 6C.
