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. 2014 Dec;351(3):654–662. doi: 10.1124/jpet.114.218339

Fig. 5.

Fig. 5.

Attenuation of late loss of paracellular claudin-5 expression by PI3K, but not AKT inhibitors. (A) Immunofluorescence analysis showed sharply defined paracellular claudin-5 expression in control cells. (B) After 2 days of 3-chloropropanediol administration, sharply defined claudin-5 expression was lost (asterisks). (C–E) Pretreatment with LY294002 (2.5–25 μM, arrows) showed a dose-dependent attenuation of the late loss of paracellular claudin-5 expression. (F–H) Loss of sharp claudin-5 expression could also be prevented by PI-828 (25 μM, arrow) (F), but not by 10-DEBC (25 μM) (G) or AKT1 (25 μM) (H). Scale bar, 25 μm.