Fig. 8.

Apoptosis and cell cycle in HT-29 cells transfected with human PXR with or without rifaximin treatment, including total apoptosis, early apoptosis, late apoptosis, and cell cycle. Human PXR and RXRα expression vectors were transfected to HT29 cells. These transfected cells were treated with dimethylsulfoxide or two different concentrations of rifaximin. Cont, cell treated with DMSO; H, cell treated with high dosage (100 μM) of rifaximin; L, cell treated with low dosage (1 μM) of rifaximin. Cells were treated in two batches (n = 3 per batch); therefore, cont1, L1, H1, cont2, L2, and H2 were labeled as listed. *P < 0.05, compared with the control group; **P < 0.01, compared with the control group; ^#P < 0.05, compared with the low dosage group.