Fig. 1.

Structures and activity of specific BMP inhibitors. (A) Previously reported structures for LDN, DMH2, and VU0469381 are included for comparison with VU0465350. (B) Representative Western blot demonstrating VU0465350 (VU5350) and 1LWY inhibit phosphorylation of SMAD1/5/8 in C2C12 cells. Concentrations of experimental compounds are provided above each lane in micromolar concentrations. Both compounds strongly inhibited SMAD phosphorylation in a dose-dependent fashion. Each experiment was performed in triplicate with P <0.01 except for 2 μM 1LWY (P = 0.018).