Fig. 5.

KLF9 does not bind to HNF4α in vitro. (A) HEK293T cells were transfected with the pGL3-CYP2D6 vector (−2455/+90), the expression vectors (HNF4α and/or 3xFLAG-tagged KLF9), and pCMV-Renilla vector. After 48 hours, dual luciferase assays were performed (triplicate experiment, mean ± S.D.; **P < 0.01 versus lane 1). (B) HEK293T cells were cotransfected with the 3xFLAG-tagged KLF9 and HNF4α expression vector. Cell lysates were prepared, and immunoprecipitation was performed using magnetic beads coated with antibodies against HNF4α (lane 1) or FLAG (lane 2). Immunoprecipitates were analyzed by Western blot. One percent of the input (lane 3) was loaded onto the gel as a control.