Table 3.
R2 and efficiency of the standard curves obtained with the modified TaqMan® Small RNA Assay.
| Muscle |
Plasma |
|||||
| R2 | Efficiency | R2 | Efficiency | |||
| mir-1 | 0.999 | 117.9 | miR-16 | 0.986 | 100.0 | |
| miR-15a | 0.993 | 110.1 | miR-20a | 0.988 | 107.0 | |
| miR-16 | 0.999 | 133.5 | miR-21 | 0.960 | 110.3 | |
| miR-21 | 0.994 | 166.6 | miR-126 | 0.993 | 94.4 | |
| miR-126 | 0.999 | 94.9 | miR-133a | 0.996 | 88.5 | |
| miR-133a | 1.000 | 99.0 | miR-146a | 0.967 | 109.4 | |
| miR-210 | 0.996 | 101.0 | miR-454 | 0.994 | 119.1 | |
| miR-221 | 0.993 | 133.2 | miR-486 | 0.998 | 96.6 | |
| miR-222 | 0.996 | 115.7 | ||||
| RNU44 | 0.999 | 100.6 | ||||
| RNU48 | 0.998 | 101.3 | ||||
R2 value is the coefficient of determination of the standard curves and the efficiency is the rate at which the polymerase chain reaction (PCR) amplicon is generated. The R2 and the efficiency were calculated by the MxPro qPCR Software.