Figure 1.

Characterization of GABA_AR subunit expression in Triton X-100 soluble membrane and detergent resistant membrane (DRM) fractions. (A) Schematic of the subcellular fraction protocol coupled with 0.5% Triton X-100 detergent extraction of insoluble membranes. Expanded details on the extraction method and detergent extraction are listed in the Methods section. (B) Characterization of synaptic and extrasynaptic proteins and GABA_AR subunits in soluble membrane and DRM fractions prepared from hippocampus of rats. Representative western blots of equal amounts of protein from each fraction using antibodies targeting the postsynaptic density protein PSD-95, the extrasynaptic membrane-associated protein TARP γ-4, and the GABA_AR α1, α4, α5, and δ subunits.