Figure 5. Nuclei were counterstained with Hoechst 33258 and observed under a fluorescence microscope. All cells were cultured for 48 h in different culture environments. Cells in the AGE-BSA group (left panel) showed bright blue dots in the nuclei, which represented nuclear fragmentation in the apoptotic cells. Cells in the BSA group (middle panel) and control group (right panel) were uniformly blue in viable cells. Magnification, 400×.