Figure 6. Effects of taurine on phosphorylated myosin. A, Effects of taurine on high-extent phosphorylated myosin, 0.02 μM myosin light chain kinase (MLCK), and 4.0 μM myosin purified from chicken gizzard smooth muscle used in the assay. B, Effects of taurine on low-extent phosphorylated myosin, 2.0 μM MLCK and 4.0 μM myosin purified from chicken gizzard smooth muscle used in the assay. Lanes 0-6 represent unphosphorylated myosin (without MLCK and taurine), high- or low-extent phosphorylated control (without taurine), high- or low-extent phosphorylated myosin with 5, 10, 20, 40, and 80 mM taurine, respectively. C, Extent of myosin phosphorylation, which was analyzed using the Gelpro software. The extent of phosphorylated myosin was calculated as percentage of phosphorylated regulatory myosin light chain of 20 kDa (MLC₂₀) in total MLC₂₀. Mono-phosphorylation was calculated as 100% phosphorylation. *P<0.05 vs high- or low-extent phosphorylated control (without taurine) (one-way ANOVA) (lane 1). MLC₂₀: unphosphorylated MLC₂₀ (20 kDa regulatory myosin light chain); p-MLC₂₀: mono-phosphorylated MLC₂₀; MLC₁₇: 17 kDa myosin essential light chains.