Figure 1.

Effects of Crotonis Fructus extracts (CEF) and croton oil (CO) on glycerol release and viability. Differentiated OP9 adipocytes were treated with various concentrations of CFE (20–50 μg/mL), CO (2–20 μg/mL), and 200 nM isoproterenol (ISO) for 12 h. Glycerol release (black bar) was determined with a glycerol quantification kit and cell viability (gray bar) was quantified with MTT assay as described in Materials and Methods. Data are expressed as the mean ± SD of four independent experiments and a percentage of vehicle (DMSO-)treated control cells (CON). ^* P < 0.05, ^** P < 0.01 versus CON.