Figure 3.
Single cell gel electrophoresis analysis (DNA Comet assay) (a) and PicoGreen assay (b). HT 29 cells were seeded in 96-well flat bottom plates at 1 × 106 viable cells/mL and were allowed to adhere at 37°C overnight. The cells were treated with the following concentrations of cytotoxic agent for 24 h: control = culture medium without Uncaria tomentosa extract or oxaliplatin; chemotherapy = oxaliplatin 20 μmol/L; Uncaria = Uncaria tomentosa extract 750 μg/mL; Uncaria + chem. = Uncaria tomentosa extract 750 μg/mL + oxaliplatin 20 μmol/L. The supernatant was removed and replaced with culture medium for over 24 h (named 48 h). Different lowercase letters represent statistically significant differences among the treatments (P < 0.05).