Figure 4.
Caspase activities. It was determined using Fluorimetric Assay Kits. HT 29 cells were seeded in 96-well flat bottom plates at 1 × 106 viable cells/mL. The cells were treated with the following concentrations of cytotoxic agent for 48 h: control = culture medium without Uncaria tomentosa extract or oxaliplatin; chemotherapy = oxaliplatin 20 μmol/L; Uncaria = Uncaria tomentosa extract 750 μg/mL; Uncaria + chem. = Uncaria tomentosa extract 750 μg/mL + oxaliplatin 20 μmol/L. Different lowercase letters represent statistically significant differences among the treatments (P < 0.05).