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. 2014 Nov-Dec;24(6):349–355. doi: 10.4103/0971-4065.132992

Figure 1.

Figure 1

Flow cytometric detection of Th1, Th2, and Treg cells in sustained remission, steroid resistant and healthy control. Stimulated peripheral blood were stained with fluorescein isothiocyanate-conjugated CD4 mAb and AlexaFluor 647 conjugated interferon-γ mAb for Th1 cells (1) and APC conjugated IL-4 mAb for Th2 cells (2) For Treg cells fluorescein isothiocyanate-conjugated CD-4 mAb, PerCP-Cy conjugated CD-25 mAb and AlexaFluor 647 conjugated FoxP3 mAb were used for staining of whole blood (3)