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. 2014 Nov 26;8:138. doi: 10.3389/fncir.2014.00138

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Copyright © 2014 Kim, Miri, Leung, Berndt, Mourrain, Tank and Burdine.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution and reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Tg[HuC:Gal4VP16; 1xK + NnlsGC3] larvae exhibit robust expression of GCaMP3 up to at least 14 dpf. (A–C) Epifluorescence images at 2 and 14 dpf of GCaMP3 expression in Tg[HuC:GCaMP3], Tg[HuC:Gal4VP16; 4xK + NnlsGC3] and Tg[HuC:Gal4VP16; 1xK + NnlsGC3]. Only Tg[HuC:Gal4VP16; 1xK + NnlsGC3] larvae exhibit robust GCaMP3 expression at 14 dpf.