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Truncation analysis of 29-1 identifies core domain responsible for fluorescence activation. The mFold-predicted secondary structure of 29-1 is presented. The borders of three truncations (T1, T2, T3, and T4) are indicated. Only T1 and T2 were able to induce fluorescence of DFHBI-1T (indicated with a green circle compared to a black, i.e., nonfluorescent circle) as measured under excess RNA conditions.
