Skip to main content
. Author manuscript; available in PMC: 2015 Mar 1.
Published in final edited form as: Kidney Int. 2013 Dec 4;85(3):611–623. doi: 10.1038/ki.2013.462

Figure 7. Lipid storage in tubular cells in mice fed a high-fat diet (HFD).

Figure 7

(a) Representative photomicrograph (original magnification ×40) showing that the vacuoles (arrow) are negative for Oil Red O Staining, (b) Representative photomicrograph illustrating cholesteryl esters with fluorescent dye Nile red staining in HFD (arrows), (c, d) Representative photomicrographs (original magnification ×1000) of semi-thin sections (original magnification ×1000) illustrating Toluidine Blue–positive dark blue vacuoles (arrow). Electron microscopy evaluation of ultrastructure of vacuoles in proximal tubules in mice treated with a HFD: presence of (e, f) enlarged clear vacuoles and (g, h) multilaminar inclusions (i: higher magnification), (j, k) Representative photomicrographs (original magnification ×1000) illustrating phospholipid accumulation in vacuolated tubule with Luxol Fast Blue in HFD (arrow, vacuoles stained in blue).