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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: Nat Cell Biol. 2014 Aug 31;16(9):852–863. doi: 10.1038/ncb3030

Figure 2.

Figure 2

Reconstitution of the hetero-octameric human augmin complex with recombinant proteins. (a-c) Size exclusion chromatography (Superose 6) elution profiles for GFP-labeled holo-complex (Hice1·GFP-hDgt6·UCHL5IP·GFP-Cep27·C14orf94 ·Ccdc5·His-hDgt3·hDgt5) (a), octamer[hDgt6Δ (433–955)] (Hice1·hDgt6 (1–432) ·UCHL5IP·GFP-Cep27·C14orf94· Ccdc5·His-hDgt3·hDgt5) (b) and octamer[hDgt6Δ (433–955), Hice1Δ (1–140)] (Hice1 (141–410) ·hDgt6 (1–432) ·UCHL5IP·GFP-Cep27·C14orf94· Ccdc5·His-hDgt3·hDgt5) (c) with peak fractions (volumes indicated) analyzed by SDS-PAGE (stained with Coomassie blue). The void volume (Vo) is indicated and absorbance (A.U.) at 280 nm is shown. (d) Analysis of the subunit composition of the augmin octamer[hDgt6Δ (433–955)] by mass spectrometry. Identities, percent sequence coverage, and number of peptides (unique and total) detected are shown.