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. Author manuscript; available in PMC: 2015 Jan 31.
Published in final edited form as: Nature. 2014 Jun 22;511(7511):616–620. doi: 10.1038/nature13393

Extended Data Figure 9. THZ1 inhibits RNAPII CTD phosphorylation and causes cell cycle arrest in non-transformed cell lines.

Extended Data Figure 9

a and b, THZ1 inhibits RNAPII CTD phosphorylation. RPE-1 (a), and BJ fibroblasts (b) were treated with THZ1 or THZ1-R for 4 hrs. Cellular lysates were then probed with antibodies against the indicated proteins. c and d, THZ1 causes cell cycle arrest in non-transformed cells. RPE-1 (c) and BJ fibroblasts (d) cells were treated with THZ1, Flavopiridol, Staurosporine, or DMSO vehicle for the indicated time periods. Cell cycle progression was analyzed following permeabilization and staining with propidium iodide. e and f, THZ1 inhibits proliferation of non-transformed cell lines. RPE-1 (e) and BJ fibroblasts (f) cells were treated with THZ1, THZ1-R, Flavopiridol, or Staurosporine for 72 hrs and antiproliferative effect was determined by Cell Titer Glo. Error bars are +/− SD.