Table 1.

Composition, particle size (P), zeta potential (Z), and maximum percentage of drug entrapped (PDE) of various batches of Chitosan-POR nanoparticle formulations as per experimental design.

F. code	Trial no.	Independent variables (coded factor levels)			Dependent variables
		X1: CS conc. (%w/v)	X2: POR conc. (%w/v)	X3: TPP conc. (%w/v)	P (nm)	Z (mV)	PDE (%)
22	1	0.04	0.025	0.02	165	36.98	62.23
24	2	0.04	0.025	0.02	174	34.57	67.15
9	3	0.006364	0.025	0.02	111	24.44	68.11
12	4	0.04	0.033409	0.02	212	33.23	65.22
3	5	0.02	0.03	0.01	170	29.22	70.11
10	6	0.073636	0.025	0.02	360	58.33	68.11
20	7	0.04	0.025	0.02	179	35.86	77.22
7	8	0.02	0.03	0.03	125	26.62	82.29
17	9	0.04	0.025	0.02	179	37.12	69.23
21	10	0.04	0.025	0.02	172	36.33	71.18
18	11	0.04	0.025	0.02	186	34.89	56.67
15	12	0.04	0.025	0.02	168	39.22	74.76
23	13	0.04	0.025	0.02	174	35.11	63.99
8	14	0.06	0.03	0.03	240	38.46	81.11
5	15	0.02	0.02	0.03	112	21.88	87.22
2	16	0.06	0.02	0.01	310	48.74	61.58
19	17	0.04	0.025	0.02	185	32.67	68.29
13	18	0.04	0.025	0.003182	255	43.11	66.22
16	19	0.04	0.025	0.02	181	28.44	73.11
4	20	0.06	0.03	0.01	340	51.11	72.89
6	21	0.06	0.02	0.03	261	45.11	76.62
14	22	0.04	0.025	0.036818	158	25.12	82.23
1	23	0.02	0.02	0.01	133	23.11	76.33
11	24	0.04	0.016591	0.02	198	27.33	60.39