Table 3.
Dose (1, 4, and 8 mg/kg/day of AmB) related in vivo hematological responses of prepared formulations against marketed and reference drug.
| Parameters | Doses (mg/kg/day) | Std. drug sol. | Fungizone | Ambisome | CS-AMB | CS-POR-AMB | CS-POR-AMB-TPP | Control | Blank |
|---|---|---|---|---|---|---|---|---|---|
| RBCs (×106/μL) | 1 | 5.21 ± 0.3∗ | 5.77 ± 0.2 | 6.16 ± 1.3 | 6.18 ± 1.8 | 7.12 ± 1.5 | 7.08 ± 0.27 | 7.96 ± 1.8 | 8.12 ± 1.2 |
| 4 | 4.11 ± 1.2 | 4.81 ± 1.3 | 5.21 ± 1.1 | 5.53 ± 1.7 | 6.78 ± 1.8 | 6.31 ± 1.6 | |||
| 8 | 3.71 ± 1.1∗ | 4.07 ± 1.3∗ | 4.14 ± 0.3 | 4.11 ± 0.41 | 5.72 ± 1.2 | 5.22 ± 1.0 | |||
|
| |||||||||
| WBCs (×103/μL) | 1 | 4.7 ± 0.3∗ | 5.1 ± 1.6 | 6.0 ± 1.1 | 5.9 ± 1.4 | 6.2 ± 0.7 | 5.8 ± 1.6 | 6.2 ± 1.1 | 5.9 ± 0.2 |
| 4 | 4.4 ± 1.3 | 4.6 ± 1.3 | 5.5 ± 1.4 | 5.7 ± 0.6 | 5.7 ± 1.2 | 5.3 ± 0.1 | |||
| 8 | 3.8 ± 0.8∗ | 4.8 ± 1.7∗ | 5.1 ± 1.8 | 4.9 ± 0.3 | 5.4 ± 1.1 | 5.2 ± 0.2 | |||
|
| |||||||||
| PL (×103/μL) | 1 | 134 ± 23 | 178 ± 11 | 275 ± 18 | 281 ± 26 | 389 ± 41∗ | 371 ± 33 | 372 ± 21 | 433 ± 33∗ |
| 4 | 106 ± 28 | 144 ± 27 | 239 ± 23 | 237 ± 1.3 | 274 ± 11∗ | 266 ± 23 | |||
| 8 | 92 ± 11∗ | 116 ± 13∗ | 191 ± 16 | 182 ± 12 | 211 ± 31∗ | 207 ± 17 | |||
|
| |||||||||
| HT (%) | 1 | 30.2 ± 2.4 | 32.1 ± 2.2 | 35.2 ± 3.1 | 34.1 ± 2.8 | 38.7 ± 3.4 | 38.2 ± 2.4 | 42.1 ± 3.1 | 41.1 ± 2.7 |
| 4 | 28.1 ± 3.1 | 30.7 ± 1.1 | 33.5 ± 2.1 | 31.1 ± 1.9 | 36.6 ± 3.1 | 35.2 ± 3.3 | |||
| 9 | 26.1 ± 2.5∗ | 29.1 ± 2.6∗ | 30.2 ± 1.7 | 29.1 ± 2.3 | 35.1 ± 1.7 | 34.1 ± 1.8 | |||
|
| |||||||||
| Hb (g/dL) | 1 | 12.1 ± 1.3 | 13.2 ± 0.3 | 13.8 ± 0.2 | 13.3 ± 0.5 | 14.6 ± 3.2∗ | 14.3 ± 1.2 | 15.2 ± 1.4 | 15.1 ± 1.2 |
| 6 | 11.2 ± 1.1 | 12.4 ± 1.4 | 12.6 ± 1.6 | 12.1 ± 1.8 | 13.7 ± 0.3 | 13.6 ± 1.6 | |||
| 9 | 10.3 ± 0.2∗ | 11.1 ± 0.2 | 11.8 ± 0.1 | 11.7 ± 1.2 | 12.8 ± 1.3 | 12.7 ± 0.1 | |||
RBCs (red blood cells); WBCs (white blood cells); Hb (hemoglobin); PL (platelet); HT (hematocrit).
∗Significant (P < 0.05, all groups compared with control). n = 6 in each group. Values are expressed as mean ± S.E.M. Data was analyzed by one-way ANOVA followed by Tukey's post hoc test.