Figure 4.

Renal morphologic injury was induced by combined dehydration for 48 hours with iodixanol (10 mL/kg) administration in 5/6 NE rats. ((a)–(c)) Representative photomicrographs of kidney injury from experimental rats treated with dehydration for 48 hours and iodixanol (10 mL/kg). (a) Representative photomicrographs of the most severe and pronounced alterations were observed in the renal corticomedullary boundary zone (the cortex and outer stripe of the outer medulla). (b) Representative photomicrographs of tubular dilation (black arrow), foamy degeneration (black arrow head), detachment of tubular cells (white arrow), and naked basement membranes (white arrow head) were observed. (c) Representative photomicrographs of proteinaceous casts (arrow) and inflammatory cell infiltration (arrow head) were observed. ((d), (e)) Representative photomicrographs of proliferation and hypertrophy (arrow) in tubular epithelial cells in 5/6 NE rats six weeks after the ablative surgery. (f) Representative photomicrographs of the renal morphology in a healthy rat. Original magnifications: ×100 ((a), (d)); ×400 ((b), (c), (e), (f)). Hematoxylin and eosin stain. Calibration bar = 20 μm.