Table 2.

List of 36 potential biomarkers identified to be significantly differentially expressed by HT-qPCR.

Number		MicroRNA	Mean Ct of DS	Mean Ct of control	ΔCts	Expression in DS relative to control	P value
1	Commonly identified by Cqs and NRQs	HSA-MIR-512-5P	30.97	33.66	−2.69	Overrepresented	0.00861
2		HSA-MIR-483-5P	31.80	33.82	−2.02	Overrepresented	0.00454
3		HSA-MIR-888-3P	30.58	34.67	−4.09	Overrepresented	0.00428
4		HSA-MIR-3676-3P	33.30	31.23	2.08	Underrepresented	0.03754
5		HSA-MIR-3918	27.66	28.39	−0.73	Overrepresented	0.02360
6		HSA-MIR-500A-5P	30.58	31.40	−0.82	Overrepresented	0.02986
7		HSA-MIR-30C-5P	27.05	28.07	−1.02	Overrepresented	0.04203
8		HSA-MIR-200C-3P	32.21	30.53	1.69	Underrepresented	0.04393
9		HSA-MIR-627	33.36	31.97	1.40	Underrepresented	0.04518
10	Identified by Cqs only	HSA-MIR-5893P	31.40	33.86	−2.46	Overrepresented	0.00283
11		HSA-MIR-181A-3P	33.55	30.79	2.75	Underrepresented	0.01864
12		HSA-MIR-362-5P	31.94	34.54	−2.60	Overrepresented	0.02495
13		HSA-MIR-518C-5P	31.35	35.04	−3.69	Overrepresented	0.01990
14		HSA-MIR-3667-5P	30.69	33.21	−2.52	Overrepresented	0.02596
15		HSA-MIR-195-3P	29.35	30.58	−1.23	Overrepresented	0.03372
16		HSA-MIR-124-5P	31.72	33.32	−1.60	Overrepresented	0.03635
17		HSA-MIR-498	34.42	31.45	2.98	Underrepresented	0.02934
18		HSA-MIR-18B-3P	30.71	33.30	−2.59	Overrepresented	0.04078
19		HSA-MIR-1249	26.72	29.20	−2.48	Overrepresented	0.04587
20		HSA-MIR-93-5P	25.76	24.76	1.00	Underrepresented	0.00944
21		HSA-MIR-20A-5P	25.04	24.27	0.77	Underrepresented	0.01837
22		HSA-MIR-4328	32.22	31.25	0.97	Underrepresented	0.02078
23		HSA-MIR-92A-3P	22.95	22.10	0.85	Underrepresented	0.02647
24		HSA-MIR-92B-3P	27.65	26.78	0.88	Underrepresented	0.02926
25		HSA-MIR-3652	30.62	31.64	−1.02	Overrepresented	0.02994
26		HSA-MIR-25-3P	24.19	23.42	0.77	Underrepresented	0.03759
27		HSA-MIR-623	33.77	31.63	2.13	Underrepresented	0.03945
28		HSA-MIR-3195	23.11	24.60	−1.49	Overrepresented	0.04885
29	Identified by NRQs only	HSA-MIR-423-5P	0.87	1.22	−0.35	Underrepresented	0.02007
30		HSA-MIR-27B-3P	1.41	0.85	0.57	Overrepresented	0.03759
31		HSA-MIR-3135a	1.56	0.66	0.91	Overrepresented	0.04044
32		HSA-MIR-486-5P	0.83	1.66	−0.83	Underrepresented	0.02598
33		HSA-MIR-3180-5P	0.71	1.17	−0.46	Underrepresented	0.04036
34		HSA-MIR-629-5P	0.81	1.62	−0.81	Underrepresented	0.04457
35		HSA-MIR-155-5P	0.93	1.89	−0.96	Underrepresented	0.04712
36		HSA-MIR-3907	0.80	1.46	−0.65	Underrepresented	0.04983

NRQs: normalized relative quantities; Cq: Ct values subtracted by background; GN: raw data, normalized to global mean data; QN: quantile normalized data; DS: Down syndrome.