Table 1.
ADH specific activity, root survival and seedling appearance after oxygen deprivation and caffeine treatment of 7‐d‐old seedlings of arabidopsis
| Duration of | ADH specific activity (U/mg protein ± SE) | Primary root tip survival 48 h after treatment (% seedlings) | Seedling appearance 48 h after treatment | |||
| oxygen deprivation | ||||||
| (h) | Wild‐type | adh‐null | Wild‐type | adh‐null | Wild‐type | adh‐null |
| 0 | 0·30 ± 0·002 | 0·001 ± 0·0002 | 100 | 100 | Green, erect | Green, erect |
| 6 | 0·40 ± 0·01 | 0·001 ± 0·0002 | 100 | 100 | Green, erect lateral root initiation and elongation | Green, erect lateral root initiation and elongation |
| 12 | 0·80 ± 0·01 | 0·002 ± 0·0003 | 100 | 50 | Green, erect increased lateral roots | Dark green, slightly wilted no lateral roots |
| 20 | 1·00 ± 0·02 | 0·001 ± 0·0001 | 100 | 20 | Green, erect increased lateral roots | Dark green, slightly wilted |
| 24 | 1·07 ± 0·02 | 0·001 ± 0·0002 | 95 | 10 | Green, erect increased lateral roots | Dark green,wilted |
| 36 | 2·03 ± 0·01 | 0·001 ± 0·0001 | 0 | 0 | Green, erect | Very dark green, wilted |
| 48 | 1·40 ± 0·03 | 0·001 ± 0·0003 | 0 | 0 | Dark green, wilted | Very dark green, wilted |
| Caffeine treatment of wild‐type (mm) | ADH specific activity after 24 h treatment (U/mg protein ± SE) | Primary root tip survival 48 h after treatment (% seedlings) | Root elongation 48 h after treatment (cm) | Seedling appearance 48 h after treatment | ||
| 0 | 0·25 ± 0·08 | 100 | 1·32 ± 0·01 | Green, erect | ||
| 2·5 | 2·2 ± 0·31 | 100 | 0·52 ± 0·01 | Green, erect | ||
| 5 | 2·9 ± 0·31 | 100 | 0·40 ± 0·01 | Light green, erect | ||
| 10 | 4·0 ± 0·93 | 0 | 0·00 ± 0·00 | Light brown, wilted | ||
ADH specific activity data are the mean ± s.e. from three independent experiments.