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Mass spectra of human holo-transferrin (79.7 kDa) formed by nESI from aqueous solutions containing 10 mM ammonium bicarbonate with (a) no additional salt added, (b) 1 mM NaCl, (c) 1 mM NaCl with 1.5% m-NBA, and (d) 1 mM NaCl with 2.5% sulfolane. Expansions of the 18–21+ charge states are shown to the right. Dashed lines indicate the calculated average m/z for each glycoform without adducts.
