Anterior migration of neural crest is accompanied by changes in the basal lamina. Wholemount heads in dorsal view at 10ss (A), 14ss (E), and 20ss (I). Boxed area show region of interest shown at higher magnification. A) Region of cells that will give rise to OP indicated by dashed outline (op). B–D) the leading edge of Sox10:GFP positive CNC (green) moves as a group surrounded by laminin (red, arrow). F–H) CNC (green) passes anterior separating the lamina of the neural tube (NT, red, arrow) and OP (red, arrowhead). J–L) CNC (green) is now flanked by laminin on both the side of the NT (red, arrow) and OP (red, arrowhead). Scale Bars: A, E, I=50µm; B–D, F–H, J–L =25µm.