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. Author manuscript; available in PMC: 2014 Nov 26.
Published in final edited form as: DNA Repair (Amst). 2014 Mar 12;20:82–93. doi: 10.1016/j.dnarep.2014.02.014

Fig. 4.

Fig. 4

Two different diffusion mechanics of TaqMutS during MMR. (A) Illustration of a 15.3 kb long DNA that contains an acceptor Alexa647 and +dT mismatch (see Fig. 2E). (B) Left: kymogram of the spatial positions of Cy3-TaqMutS donor and mismatch-associated acceptor (FRET) signals over time in the absence of ATP. Right: the diffusion coefficient and the lifetime of mismatch searching TaqMutS at various salt (NaCl) concentrations. (C) Left: kymogram of the spatial positions of Cy3-TaqMutS donor and mismatch-associated acceptor (FRET) signals overtime in the presence of ATP. Right: the diffusion coefficient and the lifetime of ATP-bound TaqMutS sliding clamp at various salt (NaCl) concentrations. (D) kymogram tracking multiple TaqMutS particles on a single 15.3 kb DNA containing a mismatch.