Figure 4. EGb761 decreased BBB permeability in Aβ_{1 –42} oligomer-induced bEnd.3 cells.

BBB permeability, evaluated by the Na-F leakage test, was assessed after incubation with 10 µM Aβ_1–42 oligomer. Cells were incubated with or without various concentrations of EGb761 for 2 h, followed by incubation with Aβ_1–42 oligomer for 24 h. Then, the absorbance of Na-F was determined by fluorescence spectrophotometry. Results are shown as the Mean±S.E.M. (*p<0.01, Aβ versus Control; #p<0.01, EGb761+Aβ versus Aβ).