Skip to main content
. 2014 Nov 26;9(11):e112254. doi: 10.1371/journal.pone.0112254

Figure 5. Activity of soluble TEVp5M and fifteen codon variants.

Figure 5

Cleavage activity of the constructed TEVp5M and variants expressed in E. coli strains BL21(DE3) (A) and Rosseta (DE3) (B) respectively. The fusion protein GST-tevS1-DAL was used as the substrate. After cleavage, the released DAL activity was measured, as described in Materials and Methods.