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. 2014 Nov 26;9(11):e113186. doi: 10.1371/journal.pone.0113186

Figure 1. c-Met siRNA transient and stable transfection.

Figure 1

(A) Sequences of c-Met-siRNAs and scramble siRNA. (B) and (C) c-Met-siRNAs were transiently transfected into SW620 cells, and the efficiency of c-Met knockdown was determined by (B) Western blot and (C) real-time PCR analyses. (D) SW620 cells were transiently transfected with c-Met-siRNA3. The cell viability was determined with an Alamar blue assay 24 or 48 h post-transfection. (E) The c-Met expression in SW620 (Ctrl), scramble-siRNA (Scr) or c-Met-siRNA3 (S3) stable knockdown cells was analyzed by a Western blot assay. c-Met expression returned to normal levels after the continuous cultivation of c-Met-siRNA3 stable knockdown cells. *P<0.05 and ***P<0.001.