Skip to main content
. 2014 Nov 26;9(11):e113186. doi: 10.1371/journal.pone.0113186

Figure 3. The effects of c-Met conditional knockdown on cell proliferation and migration.

Figure 3

(A) Effect of c-Met knockdown on cell proliferation. SW620, SW620-Scr and SW620-shRNA cells were treated with or without DOX (400 nM) for 72 h. The cells were then seeded in 96-well plates and cultivated for 1–5 days. Cell proliferation was determined using an Alamar Blue assay. The values are expressed as the mean ± SD. (B) and (C) Effect of c-Met knockdown on cell migration. SW620, SW620-Scr and SW620-shRNA cells were treated with DOX (400 nM) for 72 h. Cell migration was then determined using a transwell migration assay. (B) Representative results of the migration assay. (C) The number of migrating cells was expressed as a percentage of SW620 cells without DOX treatment. The data are shown as the mean ± SD. **P<0.01.