Table 1. Effect of rotenone and malonate on oxygen consumption1.
| Inhibitor2 | exp3 | Substrate | Effect on Oxygen consumption4 | |
| E9.5 | Rotenone | 1 | Malate/glutamate | NA |
| Succinate | ||||
| E9.5 | Malonate | 1 | Malate/glutamate | NA |
| Succinate | ||||
| E11.5 | Rotenone | 3 | Malate/glutamate | Inhibition |
| Succinate | None | |||
| E11.5 | Malonate | 3 | Malate/glutamate | Inhibition |
| Succinate | Inhibition | |||
| E13.5 | Rotenone | 3 | Malate/glutamate | Inhibition |
| Succinate | None | |||
| E13.5 | Malonate | 3 | Malate/glutamate | None |
| Succinate | Inhibition | |||
| Adult | Rotenone | 3 | Malate/glutamate | Inhibition |
| Succinate | None | |||
| Adult | Malonate | 3 | Malate/glutamate | None |
| Succinate | Inhibition |
1
All experiments were performed with cardiac tissue homogenates.
2
Concentrations used: 1 mM malonate, 2 µg/ml rotenone.
3
Number of individual experiments for each condition. E9.5: 7–9 hearts were pooled per experiment; E11.5 2–3 hearts were pooled per experiment.
4
NA–not applicable as Cx-1 and -2 substrates do not increase oxygen consumption at E9.5 and thus inhibitors have no effect.