Table 2. Comparing the changes in cortisol binding affinity of CBG mutants.
| CBG variant | Conformation | Kd (nM) | Fold change in Kd |
| wild-type | S (uncleaved) | 279±18 | |
| R (cleaved) | 734±48 | 2.6 | |
| P1-Arg + V336I | S (uncleaved) | 326±46 | |
| R (cleaved) | 3420±440 | 10 | |
| P1-Arg + V336L | S (uncleaved) | 295±44 | |
| R (cleaved) | 2420±200 | 8.2 | |
| P1-Arg + S100C-V236C | S (uncleaved) | 278±64 | |
| R (cleaved) | 1800±49 | 6.5 | |
| P1-Arg + S100C-V236C-V336I | S (uncleaved) | 297±40 | |
| R (cleaved) | 2260±320 | 7.6 | |
| P1-Arg + S100C-V236C-V336L | S (uncleaved) | 252±17 | |
| R (cleaved) | 1600±320 | 6.4 |
Dissociation constants of various forms of CBG in the S- and R-states. Each pair of results for a given CBG variant represents the Kd of the intact S-state and the reactive centre loop-cleaved R-state (larger Kd).