Figure 5.

Structure of cADO in complex with cyclopropyl carboxylic acid, 2 (PDB ID 4PG1). (A) The structure of cADO shows two molecules of 2 bound to the enzyme; one molecule directly coordinates to the di-iron center by the carboxyl group, other molecule assumes a “reverse” orientation where the carboxyl group extends into the solvent. (B) Overlay of structures of cADO with stearate bound (tan) and 2 bound (orange) illustrating large scale movement of helix 8 (for clarity the stearate molecule has been omitted from the figure).